Assessment of coronary microleakage marker capacity of three dyes

Juliana Francisca Grossi Heleno, Eduardo Nunes, Maria Ilma Sousa Côrtes, Frank Ferreira Silveira

Objective: The aim of this study was to assess the marker capacity of 2% methylene blue, 2% Rhodamine B and 5% nickel sulfate. Methods: After biomechanical canal preparation in 84 single-root pre-molar teeth extracted from human beings, the access cavities were sealed with Coltosol® and the specimens were made impermeable, except for 1 mm adjacent to the temporary sealing. The samples were immersed in the staining solutions and kept in an oven at 37 ºC for 3 and 7 days, and were submitted to thermal cycling. During this period, 300 cycles (5 ºC and 55 °C) of 30 seconds each were performed in a specific appliance with a digital programmer for temperature, time and number of cycles. Longitudinal sections of the specimens were obtained and observed under stereomicroscopic lens. Results: The statistical results by the Analysis of Variance showed a significant difference (p<0.05) among the groups and between the two time intervals assessed. Conclusion: There was greater leakage in the 7 day interval in all groups, and the Rhodamine B dye exhibited the higher mean leakage depth values in the two time intervals, followed by methylene blue and nickel sulfate.

Keywords: Dyes. Microleakage.

How to cite: Heleno JFG, Nunes E, Côrtes MIS, Silveira FF. Assessment of coronary microleakage marker capacity of three dyes. Dental Press Endod. 2012 Apr-June;2(2):30-6.

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