Antibacterial capacity of different intracanal medications on Enterococcus faecalis

Juliana Ferreira Piovesani, Alex Semenoff-Segundo, Fábio Luiz Miranda Pedro, Álvaro Henrique Borges, Alessandra Nogueira Porto Neves, Iussif Mamede Neto, Tereza Aparecida Delle Vedove Semenoff

Objective: To evaluate the antimicrobial activity of 2% chlorhexidine gel, copaiba oil, propolis extract and calcium hydroxide associated to propylene glycol on Enterococcus faecalis. Methods: Fifty single-rooted human teeth were prepared until K-file #50 and distributed into groups according to the intracanal substances. The positive control group was only propylene glycol. Subsequently, 100µL of microorganism broth was inoculated into the roots, except in the negative control group. Then the roots were placed in individual test tubes immersed in BHI and put into incubator at 37° C for 48 hours. After the turbidity of the medium, the roots were irrigated with sodium thiosulfate, filled with one of the test substances and immersed in BHI for 7 days at 37° C. Thereafter, the drugs were removed from the roots with help of K-files and abundant irrigation with sodium thiosulfate. The roots were immersed in BHI for 24 hours. After this, the tubes were analyzed by single trained examiner to the categorization of the culture medium turbidity. Conclusion: According to this methodology, it was possible to conclude that none substance was effective against Enterocossus faecalis .

Keywords: Calcium hydroxide. Chlorhexidine. Enterococcus faecalis. Intracanal dressing. Propolis.

How to cite: Piovesani JF, Semenoff-Segundo A, Pedro FLM, Borges AH, Neves ANP, Mamede Neto I, Semenoff TADV. Antibacterial capacity of different intracanal medications on Enterococcus faecalis. Dental Press Endod. 2012 Apr-June;2(2):53-8.

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