Potential sealing ability of a new propolis-based sealer used as apical plug compared to MTA

Josiane de Almeida, Roberta Fernandes Macarini, Bruna Casagrande Cechella, Ana Maria Hecke Alves, Wilson Tadeu Felippe

Objective: The main objective of this study was to evaluate and compare the sealing ability of an experimental propolis-based cement (EPC) and mineral trioxide aggregate (MTA), both as apical plug, using the glucose leakage system. Thereafter, a bacterial leakage test was employed to evaluate the EPC sealing ability. Methods: For the glucose leakage test, 30 root segments (12 mm) were randomly divided into two experimental groups (n = 15), according to the cement used as apical plug, as follows: G1 = MTA; G2 = EPC. Root segments were prepared and the amount of glucose leakage was measured, following an enzymatic reaction, and quantified by a spectrophotometer. Data were analyzed by means of Student’s t-test (p < 0.05). For the bacterial leakage test, another 15 teeth were subject to apexification procedures, filled with EPC and mounted in a specific apparatus. The number of leaking specimens was observed weekly for 70 days. Results: EPC was significantly superior to MTA (p < 0.001). The EPC sealing ability was 100% and 60% efficient in glucose and bacterial leakage tests, respectively. Conclusions: The experimental propolis-based cement used as apical plug promoted superior sealing ability when compared to MTA, and excellent performance in both leakage systems.

Keywords: Apexification. Dental leakage. Glucose. Enterococcus faecalis. Propolis.

How to cite: Almeida J, Macarini RF, Cechella BC, Alves AMH, Felippe WT. Potential sealing ability of a new propolis-based sealer used as apical plug compared to MTA. Dental Press Endod. 2015 May-Aug;5(2):20-5. DOI: http://dx.doi.org/10.14436/2358-2545.5.2.020-025.oar

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