Analysis of Bifidobacterium dentium and Fusobacterium nucleatum biofilm formation on different substrates by confocal laser scanning microscopy
Raquel Zanin Midena, Paulo Henrique Weckwerth, Carolina Yumi Hoshino, Fernanda Conti Blasizza e Flaviana Bombarda De Andrade
Objective: This study aimed to evaluate the influence of different substrates (bovine dentin blocks and glass blocks) on biofilm development of F. nucleatum and B. dentium by confocal laser scanning microscopy (CLSM). Methods: A 24-well culture plate were used to induce biofilm on substrates with a strain of F. nucleatum ATCC 25586 and a strain of B. dentium ATCC 27534 during seven days. After the growth induction period, specimens were stained with the Live/Dead technique and analyzed by CLSM. Results obtained by CLSM were analyzed by the bioImage_L software. Results: All results were analyzed by a nonparametric test (p<0.05). Biofilm formation occurred in all experimental groups. The total biovolume and the percentage of viable bacteria in biofilm of B. dentium on dentin and glass blocks did not show any statistic difference. Biovolume of viable bacteria did not show any difference between the substrates in biofilm of F. nucleatum on dentin and glass blocks. The total biovolume showed better results in biofilm formed on dentin blocks. B. dentium and F. nucleatum are capable of forming biofilm in all studied substrates. Conclusion: In the adopted methodology, the type of substrate influences the characteristics of the produced biofilm. Dentin blocks are more adequate to form biofilm of the microorganisms studied.
Keywords: Biofilm. Confocal microscopy. Bifidobacterium dentium. Fusobacterium nucleatum.
How to cite: Midena RZ, Weckwerth PH, Hoshino CY, Blasizza FC, Andrade FB. Analysis of Bifidobacterium dentium and Fusobacterium nucleatum biofilm formation on different substrates by confocal laser scanning microscopy. Dental Press Endod. 2016 Jan-Apr;6(1):49-55. DOI: http://dx.doi.org/10.14436/2358-2545.6.1.049-055.oar
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