Antimicrobial activity of calcium silicate-based sealer containing antibiotics, tested by means of radial diffusion and minimum inhibitory concentration

Hernando Valentim da Rocha Júnior, Francisco Orlando Giraldi Neto, Rafaela Pignatti de Freitas, Marco Antonio Húngaro Duarte, Guilherme Ferreira da Silva, Paulo Henrique Weckwerth.

Introduction: Replacement of Portland cement by MTA has been recommended in clinical practice due to similarity between their composition, as well as mechanical and biological properties. Adding antibiotics to the cement would provide clinical advantages, provided the antibiotic drug be released before and after the setting of cement. Objective: The aim of this study was to assess antimicrobial activity by diffusion and minimum inhibitory concentration (MIC) of pure Portland cement and Portland plus radiopacifier, with addition of amoxicillin and ciprofloxacin, relative to five microorganisms: S. aureus, P. aeruginosa, E. coli, E. faecalis and C. albicans. Methods: For the diffusion test, 30 Petri dishes with Muller-Hinton agar were used, six of which had wells where cements were introduced immediately after manipulation and after a 24-hour setting. For MIC testing, 120 test tubes containing 10ml of brain heart infusion (BHI) broth were prepared, and divided into four groups. Each group was cultured with a fresh inoculum of each organism separately. Tubes were incubated at 37°C and turbidity was measured after 24h. Results were assessed by ANOVA analysis of variance and comparison of groups carried out by Tukey’s test, with 5% significance. Results: Portland cement showed antimicrobial activity only against C. albicans. Amoxicillin showed a slight antibacterial effect, while ciprofloxacin inhibited bacterial growth at all concentrations tested. Conclusion: Addition of ciprofloxacin to Portland cement in order to enhance antimicrobial activity appears to be safe; however, results do not support such clinical application, thus requiring further studies to be performed.

Keywords: Root canal treatment. Local antibiotics. Antimicrobial activity tests. Calcium silicate-based sealer

How to cite: Rocha Júnior HV, Giraldi Neto FO, Freitas RP, Duarte MAH, Silva GF, Weckwerth PH. Antimicrobial activity of calcium silicate-based sealer containing antibiotics, tested by means of radial diffusion and minimum inhibitory concentration. Dental Press Endod. 2017 Jan-Apr;7(1):60-5. DOI: http://dx.doi.org/10.14436/2358-2545.7.1.060-065.oar

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