Influence of gel irrigant agents in oral biofilm

Bruno Cavalini Cavenago, Samuel Lucas Fernandes, Fábio Del Mônaco Avelar, Rodrigo Ricci Vivan, Marco Antonio Hungaro Duarte

Introduction: The aim of this study was to evaluate the antimicrobial activity of 1% sodium hypochlorite, 2% chlorhexidine and 24% EDTA, all in the gel form on oral biofilm. Methods: Sterile bovine dentin blocks were allocated on an intraoral device that was used by a volunteer for 3 days. After biofilm formation, the blocks were immersed in 100 μl of evaluated substances, for 5 minutes. After treatment, the samples were stained with 50 μl of a solution of SYTO 9 / propidium iodide and evaluated in a confocal microscope, yielding 50 images per group immediately after removing the antimicrobial agent. Data were analyzed by Kruskal- Wallis and Dunn’s tests (α = 0.05). Results: Statistical differences between experimental groups and the control were verified. The 1% sodium hypochlorite was more effective than other evaluated substances (p < 0.05). The 2% chlorhexidine significantly reduced the percentage of live cells, in comparison to EDTA gel (p < 0.05). Conclusions: The irrigant agents used in this study showed no ability to dissolve induced in situ biofilm. However the sodium hypochlorite gel showed better results than chlorhexidine and EDTA.

Keywords: Biofilms. Dentin. Microscopy, confocal.

How to cite: Cavenago BC, Fernandes SL, Avelar FDM, Vivan RR, Duarte MAH. Influence of gel irrigant agents in oral biofilm. Dental Press Endod. 2017 May-Aug;7(2):21-5. DOI: https://doi.org/10.14436/2358-2545.7.2.021-025.oar

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