A histological assessment of dentine, after the clinical removal of caries in extracted human teeth

Danielle Alves de oliveira, João Carlos Gabrielli Biffi, Camilla Christian Gomes moura, Eliseu Álvaro Pascon

Introduction: Despite the evolution in the strategies used to prevent and treat dental caries, no consensus exists regarding the relationship among caries depth and bacterial contamination. Objectives: To analyze the depth of the carious lesion after clinical removal of carious dentin, and assess the presence, location and distribution of microorganisms in the dentinal tubules, in different degrees of the lesion depth. Methods: 20 carious human premolars were evaluated to determine the relationship among bacterial invasion and clinical carious degree using Brown and Brenn’s bacterial stain. The data were subjected to Pearson’s correlation coefficient. Results: The correlation between the number of microorganisms found within the different caries degrees was considered slightly positive (r = 0.038). No correlation between the distribution and the location of microorganisms in different caries degrees was verified. Conclusion: The presence of microorganisms in critical areas, such as the enamel-dentin junction and in the deeper cavity floor suggests the influence of anatomical characteristics on caries pathology

Keywords: Dental caries. Dentin. Microorganisms. Radiographic exam.

How to cite: Oliveira DA, Biffi JCG, Moura CCG, Pascon EA. A histological assessment of dentine, after the clinical removal of caries in extracted human teeth. Dental Press Endod. 2011 Oct-Dec;1(3):79-87.

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